Abstract

PHYTOCHEMICAL EVALUATION AND GC-MS ANALYSIS OF WHOLE PLANT EXTRACT OF SEIDENFIA RHEEDII (SW.) SZLACH. (ORCHIDACEAE)

Ruthisha P. K.*, Ratheesh Chandra P. and Dr. K. M. Khaleel

Abstract

The present study investigates the qualitative and quantitative evaluation of the major bioactive constituents of medicinally important plant Seidenfia rheedii (Sw.) Szlach. in its aqueous, ethanol, chloroform, petroleum ether extract of whole plant. Carbohydrates, proteins, aminoacids, Saponins, flavonoids,terpenoids, glycosides, tannins, alkaloids, phenols were present in the sample. Carbohydrate concentration is 82.6mg/g. Alkaloid concentration is 0 .02mg/g. Phenolics concentration is high in ethanol extract and is 109mgGAE/g. Flavonoid concentration is high in water extract and is 214mgRE/g. The percentage yield of plant extract is high in ethanol extract and is 4.02%. GC-MS analysis of chloroform extract of the plant shows 2- Chloro-5,5-dimethyl-1-phenyl-3-hexen-1-ol with Peak area 20.12 was the major compound. GC-MS analysis of Petroleum ether extract shows the major compounds Octadecane, 6- methyl- with peak area 26.52 and Oxalic acid, allyl hexyl ester with peak area 14.18.

Keywords: Phytochemical evaluation, Orchidaceae, Seidenfia rheedii, GC-MS analysis.