Abstract

SARCOCYSTIS SPP. IN RELATION TO NON-SPECIFIC AND RHEUMATOID ARTHRITIS DISEASES

Huda Ghanim Dakhil*, Basim Hashim Abdallah1 and Fawzia Ali Abdallah

Abstract

The primary objective of this study was to detect antigenicity of Sarcocystis extract by ELISA test in order to facilitate development of a diagnostic tool for exploring the relation of non- rheumatoid and rheumatoid arthritis with Sarcocystis spp. Sera from control rabbits in order to determine the cut-off values of this test, the best method of inoculation and proper antigen concentration that lead to variable antibody respons representative by variable values of optic density, cut-off levels were considered to be 0.125 for the ELISA. The lower density values which was observed in control rabbits. All of the intramuscular inoculated rabbits serum (100%) were considered moderately positive. The highly positive cases were associated with subcutaneous inoculated rabbits sera (50%) showed high optical density value (0.2) while other (50%) animals had moderate values. Made this method to detection of parasite-specific antibodies in ELISA, soluble somatic extracts of bradyzoites in PBS was used as antigen in this assay. This is the first study for the evaluation of the ELISA for detecting antibodies to Sarcocystis spp. in Rhematoid arthritis patients in Iraq. Samples of human was performed on 51 patients attending Misan province Hospitals and private laborataries, Twenty sera from healthy individuals were tested, in order to determine the cut-off values of the ELISA, cut-off levels were considered 0.95 for the ELISA. The highly positive cases were associated with Rheumatoid arthritis patients sera samples (6.6%) showed high absorbance values (2.49- 2.70). The over all seropositivity among the examined serum samples was 29.4% When calculated for each group separately, it was 33,3% in the rheumatoid arthritis patients and 23.8% in non- specific arthritis patients.

Keywords: Sarcocystis spp. In Iraq, rheumatoid arthritis and non- specific arthritis.