Abstract

MOLECULAR DETECTION OF RPOB GENE FROM ACINETOBACTER BAUMANNII ISOLATED FROM DIFFERENT CLINICAL SOURCES

Riad A. Dellol and Alaa S. Hamzah*

Abstract

This study carried out to detection of rpoB gene from Acinetobacter baumannii isolated from different clinical sources in Baghdad governorate. A total (200) clinical samples were collected from different clinical sources were (50) samples from urine, (50) samples from wound, (50) samples from sputum and (50) sample from otitis infections. After identification by routine methods and confirmation by using VITEK- 2 Compact system, the results showed found (20) isolates of Acinetobacter baumannii from (200) clinical samples in percentage (10%). Antibiogram of these isolates were carried out against five antibiotics and the result showed high multidrug resistant. DNA extraction of all isolates extracted by specific kit, then amplification of rpoB gene by specific primers take place and the results showed about 80% of these isolates contain rpoB gene.

Keywords: rpoB gene, Molecular detection, clinical sources.