Abstract

EFFECT OF SALINITY STRESS AND DETECTION OF AFFECTED PHYTOCOMPONENTS ON OCIMUM SANCTUM

Indranil Sarkar and Soma Patnaik*

Abstract

Current study focuses on analysis of Ocimum sanctum plantlets for their response towards salinity stress. Six-week old plantlets of Ocimum sanctum (Lakshmi variety) were used for the experiment. These plantlets were treated with different concentrations of salt and post 35 days of treatment their growth parameters were compared with the control samples The GC-MS analysis of control samples and saline treated plants revealed three major compounds being largely affected. These were identified as ricinolsaeure, tetracontane & 9, 19- cyclolanostan-24-one, 3-acetoxy-25-methoxy. Percentage concentration in terms of area measured in GC-MS chromatograms showed that ricinolsaeure in 100mM and 200mM treated samples were 15.86% and 14.34% respectively, while in control it was 9.85. There was minimalistic difference in the percentage concentration of tetracontane in control sample and sample treated with 100 mM saline solution whereas it was found to be reduced in the sample treated with 200 mM saline solution. Significantly 9,19-cyclolanostan-24-one, 3-acetoxy-25-methoxy was found to be absent in the control sample whereas in 100mM & 200mM saline treated samples the concentrations were 8.63% and 21.5% respectively. The results show that control plantlets were healthier in terms of shoot length, root length and number and unit of leaves than the saline affected plants and the phytocomponents are significantly affected by salinity stress.

Keywords: Ocimum sanctum, GC-MS, Ricinolsaeure, Tetracontane, 9,19-cyclolanostan- 24-one, 3-acetoxy-25-methoxy.