Abstract

PHYTOCHEMICAL ANALYSIS AND IN VITRO ANTIOXIDANT ACTIVITY OF LEAVES OF ANDROGRAPHIS ECHIOIDES AND SEEDS OF SPERMACOCE HISPIDA

Shreen Farhana A.J. and Venkatalakshmi P.*

Abstract

The present study has been carried out to evaluate the preliminary phytochemical screening and antioxidant activity of the ethanolic extracts of leaves of Andrographis echioides and seeds of Spermacoce hispida. The phytochemical screening of dry powder of Andrographis echioides depicts the presence of flavonoids, alkaloids, sugar, etc. and Spermacoce hispida depicts the presence of quinone, flavonoids, alkaloids, etc. In vitro antioxidant activities have been investigated by using DPPH scavenging assay, Reducing power assay, Nitric oxide (NO) scavenging assay and ABTS cation decolourization assay. The results reveal that the ethanolic extract of leaves of Andrographis echioides has DPPH radical scavenging and Nitric oxide scavenging activities whereas the seeds of Spermacoce hispida possesses potent Reducing power and ABTS cation decolourization activities.

Keywords: ABTS scavenging assay, Andrographis echioides, Antioxidant activity, DPPH radical scavenging assay, Spermacoce hispida.