Abstract

THE ANTI-PROLIFERATIVE ACTIVITY OF VITIS VINIFERA LEAVES OF WATER EXTRACT ALONE AND IN COMBINATION WITH DOXORUBICIN AGAINST LIVER CANCER CELL LINE.

Haitham M. Kadhim, Hayder B. Sahib, Zainab Khaliad Ali* and Hamid Naji

Abstract

The aim of this study was to investigate the capability of Vitis vinifera leaves of water extracts alone and in combination with doxorubicin to inhibit HepG2 liver cancer cell line proliferation and to identify the possible mechanism of action. The anti proliferative activity of the water extracts tested by 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide (MTT) assay on liver cancer cell line (HepG2). Dose response relationship was shown. Significant cytotoxic activity of water extracts has identified on HepG2, the IC50 was 20.8μg/ml. When in combination with doxorubicin Dose response relationship was shown. No significant cytotoxic activity of the water extracts combination; the IC50 was 31.5μg/ml. These finding showed that water extracts combination did not show any cytotoxic action at the applied dose, so no toxic effect against the HepG2 cell line can be expected in vitro (contrast action).

Keywords: Vitis vinifera, water, doxorubicin, HepG2.