Abstract

ASSESMENT OF THE VARIANTS PRODUCED THROUGH TISSUE CULTURE TECHNIQUES IN WITHANIA SOMNIFERA (L.)Dunal BY RAPD PCR METHOD

Indrani Trivedi*, Vijay Kumar Jha, Pankaj Kumar, Bishun Deo Prasad, Sanjeev Kumar Ambasta, Birendra Prasad, Maheshwar Prasad Trivedi and Upendra Kishore Sinha

Abstract

Withania somnifera (L.)Dunal is regenerated from callus cultures on MS medium supplemented with BAP and 2,4-D.The random amplified polymorphic DNA (RAPD) assay was performed to assess the genetic diversity among Withania somnifera (L.)Dunal regenerant and its mother plant. The 12 decamer primers were used in this study. Out of 158 bands obtained 29 were polymorphic. The combination of RAPD data and the simple matching coefficient grouped the species in clusters. The data derived from RAPD marker helped in a comprehensive understanding of the genetic relationship between Withania somnifera (L.)Dunal variants.

Keywords: Withania somnifera, RAPD, Regenerants, somaclonal variation.