Abstract

STUDY OF EFFECTIVENESS AND RESISTANCE OF VARIOUS TOOTHPASTE ON MOUTH MICROBIAL FLORA

Shishir Tiwari, Dr. Shweta Sao* and Antu Kurrey

Abstract

There are various toothpastes, which are used for brushing the teeth in different parts of the world. Several studies have been reported on the resistance and effectiveness of toothpastes on oral bacteria. This study was conducted to evaluate the resistance and effectiveness of various class of toothpastes on oral microflora. The present study was conducted to assess resistance and effectiveness of various concentration of toothpastes on oral microflora. The well method was used to test the resistance on oral bacteria. Wells were prepared on blood agar and Mueller Hinton agar plates with the help of punch having 6mm diameter. The plates were left for 1h at room temperature and then incubated at 37°C for 24hr and examined for zone of inhibition. Red toothpaste shown no zone of inhibition with Enterococcus and Streptococcus in any concentration and with staphylococcus, observed zone of inhibition in 100% and with Lactobacillus observed zone of inhibition in 100% and 5% concentration. Chemical toothpaste shows no zone of inhibition with Staphylococcus and Lactobacillus in 10% concentration and observed higher zone of inhibition with Streptococcus in 100% concentration. Herbal toothpaste shows significantly highest zone of inhibition with Enterococcus in 100% concentration. Both herbal and chemical toothpastes had sensitivity against Streptococcus, Enterococcus, Lactobacillus and Staphylococcus bacteria, and powder toothpaste have resistaance against Lactobacillus and Staphylococcus bacteria in only with 100% and 5% concentration. Resistance was significantly higher in herbal toothpaste than chemical toothpaste.

Keywords: Streptococcus, Enterococcus, Lactobacillus, Staphylococcus, Microflora, Resistance, Blood Agar, Mueller Hinton Agar, Zone of inhibition. Toothpaste.