Abstract

DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF OFLOXACIN AND ORNIDAZOLE IN COMBINED DOSAGE FORM USING SIMULTANEOUS EQUATION METHOD

Farrel Lisa Gauncar* and Sachi S. Kudchadkar

Abstract

A simple, precise UV spectrophotometric method has been developed for determination of ofloxacin and ornidazole in its tablet dosage form by using distilled water as a solvent. The absorption maximum (λ max) was observed at 287 nm and 318 nm respectively. The method obeys beer lamberts linearity in the range of 2-12 ug/ml for ofloxacin and 5- 30 ug/ml for ornidazole with a value of correlation coefficient (R2) 0.999 for both the drugs at their respective wavelength. Assay was performed on two different brands of marketed formulation. Precision studies were carried out as per ICH guidelines Q2 R1and were found to be within the limits NMT 2 % concluding that the method is precise. The proposed method can be used for routine purity testing in bulk and combined dosage form.

Keywords: Ofloxacin, Ornidazole, OFX, ORN, simultaneous equation method, UV spectrophotometry.