Abstract

IN VITRO RESPONSE OF CROTALARIA HEBECARPA (DC.)RUDD.

Avula Srinivasa Rao, Shaik Babu Saheb, Luay Kadhim Hanoon and Kokkanti Mallikarjuna*

Abstract

Crotalaria hebecarpa is a small unexplored prostate herb belonging to the family Fabaceae. Though the identity and distribution of the plant is known, but its phytochemical and nutritional composition and their biological activities are not known. Since nothing is known about this plant, establishment of in vitro plants and bio mass enables us to use it for phytochemical and pharmacological exploration. In this paper, we for the first time are reporting the in vitro response of seeds, shoots and roots cultured on MS medium supplemented with different phyto hormones. The seeds cultured on MS medium supplemented with KN (4mg/l) produced shoots (Mean length, 9.23cm) while on Zeatin supplemented medium produced shoots and roots with mean length of 10cm and 7cm respectively. Maximum percentage of callus from leaf explants was observed at 3 mg/l of NAA (90%) and from roots at 2 mg/l Zeatin (95%). Maximum number of multiple shoots from callus was observed at 5mg/l BA (7.01±0.21) and at 4 mg/l of KN (5.45±0.64). The work in continuation to this preliminary report needed to be conducted to complete micro propagation process and to establish cell or cell suspension system for secondary metabolite production.

Keywords: Crotalaria hebecarpa, phyto hormones and in vitro studies.