Abstract

AN ASSESSMENT OF IN VITRO CYTOTOXICITY OF CYPERMETHRIN ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES USING MTT ASSAY

Dayashankar Gautam*, Reena Chauhan, Archana Patel, Nandini Lodhi

Abstract

Cypermethrin, an analogue of pyrethrins, is a pesticide used in pest control. This study dealt with the investigation of in vitro cytotoxicity of Cypermethrin on human peripheral blood lymphocytes (PBL) at 2 hrs and 18 hrs exposure. Cytotoxicity of Cypermethrin was assessed on human peripheral blood lymphocytes using MTT assay. Cells were treated with 1μg/ml, 2.5μg/ml, 5μg/ml, 7.5μg/ml and 10μg/ml of pesticide. The cell viability at 1μg/ml on 2hrs exposure was found to be 67.09±0.07% while it was 87.10±0.25 % on 18hrs exposure group. A dose related drop in viability in the survival of lymphocytes was found when they were treated with different concentrations of Cypermethrin. Cell viability% on 18hrs exposure was found to be higher than that of 2 hrs exposure group. Less mortality in 18hrs exposed cells might be due to self-recovery process of cells.

Keywords: Cytotoxicity, Human peripheral blood lymphocytes (PBL), MTT assay, Cell viability.