Abstract

ISOLATION OF GENOMIC DNA AND RESTRICTION ENDONUCLEASE ANALYSIS IN CESTODE PARASITE, RAILLIETINA ECHINOBOTHRIDA INFECTING THE INTESTINE OF THE COUNTRY FOWL (GALLUS DOMESTICUS)

J. Manju Bashini*, S. Pandiammal and P. Senthilkumaar

Abstract

Considerable economic loss occurs due to helminth parasites in country fowl throughout the world are. Though less emphasis has been placed on utilising DNA elements to study cestodes of veterinary importance, correct diagnosis is, nevertheless, critical to proper treatment and control. These are able to induce structural, biochemical and immunological changes in the host like inappetence, diarrhoea, dull hair coat and ostertagiosis. At present, control is almost exclusively based on anthelmintics. Hence, Genomic DNA analysis plays a vital role in characterizing and classifying cestode parasites. The present study revealed restriction digestion analysis of genomic DNA of Raillietina echinobothrida reference isolates collected from country chicken “Gallus domesticus” from Zambazaar market, Triplicane, Chennai (Tamil Nadu) using 9 restriction endonucleases enzymes namely HindΙΙΙ, EcoRΙ, BamHΙ, BglΙ, HaeΙΙΙ, XhoΙ, SfiΙ, NheΙ and HinfΙ revealed some differences in the restriction profile of DNA in the chick isolates. Lambda HindIII marker was used to compare the genomic DNA bands of R. echinobothrida formed in the gel. R. echinobothrida digested with the enzymes like EcoRΙ, BamHΙ, BglΙ, HaeΙΙΙ and XhoΙ compared with 23kb of lambda HindIII marker and most of them showed streaky and smeared formation of DNA and SfiΙ, NheΙ and HinfΙ showed no bands.

Keywords: Raillietina echinobothrida, genomic DNA analysis, restriction enzymes.