Abstract

NOVEL SIMULTANEOUS AND SECOND ORDER DERIVATVE METHOD DEVELOPMENT OF DOXOFYLLINE AND SALBUTAMOL SULPHATE IN PURE AND FIXED DOSE COMBINATION BY UV – SPECTROPHOTOMETRY

Dr. G. Abirami* and Dr. T. Vetrichelvan

Abstract

Two novel simple, rapid, precise and reproducible UV spectrophotometric methods has been developed for Simultaneous estimation of two component drug mixture of Doxofylline (DOX) and Salbutamol (SAL) in bulk and combined tablet dosage form. The literature review reveals that there is no derivative method was developed for this combination of drugs, hence this method was developed. First method employs simultaneous equation method using 274nm (λ max of DOX) and 224 (λ max of SAL) as two wavelengths for estimation. The second method involves second order derivative spectroscopic method, the wavelength used were 233nm for DOX (zero crossing for SAL) and 229nm for SAL (zero crossing for DOX). For the two methods distilled water was used as solvent. Linearity was observed in the concentration range of 5- 25 μg/ml or DOX and 5- 25 μg/ml for DOX. The % recovery was found in the range of 99.64-100.07 for DOX and 98.48-100.55 for Salbutamol. The developed method was validated statistically by recovery studies. The % RSD value was found to be less than 2. Thus the proposed method was simple, precise, economic, rapid and accurate and can be successfully applied for simultaneous determination of Salbutamol in bulk and combined tablet dosage form.

Keywords: Salbutamol sulphate, Derivative spectrophotometer UV detection.