Abstract

ANTIDIABETIC EFFECT OF ETHYL ACETATE EXTRACT OF ASPARAGUS RACEMOSUS WILLD ON HIGH –FAT DIET AND STREPTOZOTOCIN INDUCED TYPE 2 DIABETIC RATS

Gopala Krishnan R.*, Vadivelan R., Arun T. and Sandhya Ch.

Abstract

Background: Diabetes is a costly disease with high financial burden. This study is to treat the diabetes using natural resources. Therefore, the present study was designed to investigate the ethyl acetate extract of Asparagus racemosus Willd for alleviating the symptoms of diabetes mellitus. Methods: After induction of the diabetic rat model T2DM using high fat diet (HFD)/streptozotocin (STZ),thirty male Wistar albino rats were assigned into normal control, T2DM untreated, and ethyl acetate extract of Asparagus racemosus Willd treated diabetic rat groups. T2DM induction through 3weeks of high fat diet (HFD) intervention was followed by single low dosage of STZ (30mg/kg dissolved in 0.1 mol/L citrate buffer at pH 4.5,i.p). Result: Diabetic rat models showed a significant increase in blood glucose level as compared to the treated animals, which were determined by OGTT. T2DM rats have decreased levels of liver glucose 6 phosphate dehydrogenase (G6PD), reduced glutathione (GSH), nitric oxide (No), and antioxidant enzymes. Furthermore, the present study showed hypoglycemic, hypolipidemic, and antioxidant activity of the ethyl acetate extract of Asparagus racemosus Willd as confirmed by its ability for ameliorating most of the alteration caused in the study parameters of diabetic rats. Conclusion: The ethyl acetate extract of Asparagus racemosus Willd may be useful as therapy against oxidative stress and liver damage in type2 diabetes mellitus and is therefore recommended for further studies.

Keywords: T2DM, Ethyl acetate extract of Asparagus racemosus Willd, hyperglycemia, Lipid profile, Liver toxicity, Oxidative stress.