Abstract

HEPATOPROTECTIVE EFFICACY OF OCIMUM CANUM SIMS. ON ISONIAZID INDUCED HEPATOTOXICITY

Dr. Radhika J.*, Jothi G., Nivethetha M., Vijayaroslin L. and Yuvarani S.

Abstract

Liver is the vital organ involved in metabolism and independently involved in many biochemical functions. Liver is the key organ and the principal site where the metabolism of carbohydrate, proteins, lipids and detoxification take place. It is a fifth most common cause of death. The manifestation of drug induced hepatotoxicity is highly variable ranging from asymptomatic elevation of liver enzymes to fulminant hepatic failure. Ocimum canum Sims a large succulent herb with aromatic leaves is known to possess carminative, diaphoretic and stimulant properties. The present study was designed to evaluate the hepatoprotective effect of the aqueous extract of Ocimum canum Sims on isoniazid induced hepatotoxic rats. Wistar strain of albino rats were divided into 6 groups with 6 rats each, group1served as normal control, group 2 served as disease control (54 mg of isoniazid /kg BW p.o once daily for a period of 21 days, groups 3,4&5 were induced with isoniazid and treated with aqueous extract of Ocimum canum Sims. 100, 200, 300mg/kg bw orally for 21 days, group 6 were induced isoniazid and treated with std drug silymarin (50mg/kg BW for 21 days orally). After the experimental period Aspartate transaminase (AST), Alanine transaminase (ALT), Alkaline phosphatase (ALP), Gamma Glutamyl transferase (GGT), Triglycerides, Bilirubin, Protein, Cholesterol, HDL, LDL, VLDL, liver glycogen, antioxidant status were analysed. Disease control rats showed increased levels of ALT, AST, ALP, Bilirubin, LPO and the levels of GSH, SOD, Protein, GPx, Cholesterol, Glycogen, triglycerides were significantly decreased when compared to normal control rats. The administration of aqueous extract of Ocimum canum Sims, lead to the restoration of the normal functioning of the liver. The above results reveal the hepatoprotective potentials of Ocimum canum Sims.

Keywords: Hepatic failure, Ocimum canum Sims, Isoniazid, Silymarin, Hepatoprotective.