Abstract

HPLC METHOD FOR THE DETERMINATION OF TRANSDERMAL DRUG DIFFUSION OF KETOPROFEN

Deep Kotal* and Dr. Rama Bukka

Abstract

A simple, isocratic and sensitive high performance liquid chromatographic method was developed for the estimation of Ketoprofen during transdermal diffusion studies across porcine ear epithelium. A reversed-phase (C18) column was used with UV detector at 260 nm. Selected mobile phase contained a mixture of Acetonitrile and double distilled water (40:60 v/v), pH adjusted to 3 using 1% ortho-phosphoric acid solution. Calibration curve showed good linearity over the concentration range of 5-50 μg/ml in ear epidermis permeates of pH 7.4 phosphate buffer (Blank permeates). The applicability of the method was demonstrated by the analysis of porcine ear epithelium permeated samples of Ketoprofen in a diffusion study. The steady state permeability Flux (J) of Ketoprofen through ear epidermis using 5mg and 10mg of Ketoprofen solution in donor cell of diffusion study was found to be 0.0002 μg/sqcm/hr and 0.0006 μg/sqcm/hr respectively.

Keywords: RP-HPLC, Flux, Diffusion study, Ketoprofen.