Abstract

ANTIOXIDANT ACTIVITY OF METHANOLIC SEED COAT EXTRACT OF ENTADA PURSAETHA DC

S. Valarmathi* and K. Raju

Abstract

The antioxidant activity of the crude methanolic extract of the seed coat of Entada pursaetha, was investigated. The total phenolic composition of methanolic extract was calculated to be 5.6mg catechol equivalents/g of sample. Antioxidant activity of the extract was evaluated on the basis of ability of scavenging free radical and hydroxyl radical with the IC50 values 2.15mg/ml and 1.005mg/ml respectively. Total antioxidant capacity of crude plant extract was found to be 1.525mg ascorbic acid equivalents at 250μg/ml extract concentration. The reducing power of the extract increased dose dependently and the extract reduced the most Fe 3+ ions to the extent less than the standard ascorbic acid.

Keywords: Antioxidant, Methanolic Extract, seed coat, Entada pursaetha, and Kolli hills.