Abstract

MODULATORY ROLE OF AZIMA TETRACANTHA LEAVES EXTRACT ON CARBON TETRACHLORIDE INDUCED OXIDATIVE STRESS IN RATS.

K. Saleem*, T. Nargis Begum and M. H. Muhammad Ilyas

Abstract

Current hypothesis favour the concept that lowering oxidative stress can have a health benefits. In this study modulatory effect of ethanolic extract of Azima tetracantha was investigated using carbon tetrachloride (CCl4)-induced oxidative stress as the experimental model. The oxidative stress rats were administered different doses (100, 200 and 400mg/ Kg BW) of Azima tetracantha leaves extract. Malondialdehyde (MDA) is a product of lipid peroxidation in CCl4 – intoxicated rats was evidenced by a marked increment in the levels of thiobarbituric acid reactive substances (TBARS) and also a distinct diminution in glutathione (GSH) content in the liver. In CCl4 + Azima tetracantha treated rats these biochemical parameters attained an almost normal as dose dependent manner. The decreased activity of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and non enzymatic antioxidant, such as glutathione (GSH), vitamin C and vitamin E in CCl4 intoxicated rats were observed. On treatment with different doses (100, 200 and 400mg/ Kg BW) of Azima tetracantha leaves extract increased in the activity as dose dependent manner. Among the various doses, 400mg/kg has potential activity than other doses. From these results, it was suggested that lipid peroxidation and oxidative stress elicited by CCl4 intoxication had been nullified due to the effect of Azima tetracantha.

Keywords: Antioxidant enzymes, Oxidative stress, Carbon tetrachloride, Lipid peroxidation, Azima tetracantha.