Abstract

ALPHA AMYLASE AND ALPHA GLUCOSIDASE INHIBITORY ACTIVITY OF AMALAKI RASAYANA-AN AYURVEDIC FORMULATION

Dr. Prashant B. Bedarkar*

Abstract

Introduction- Powder of fruit pulp of Indian gooseberry (Amalaki) levigated multiple times with its juice i.e. Amalaki swarasa bhavita Amalaki churna (ASBAC) is commonly regarded as Amalaki Rasayana (AR) is recommended in Ayurvedic classics and practiced in the management (treatment, prevention of complications) of Madhumeha (Diabetes). Alpha amylase and alpha glucosidase activities of Amalaki Rasayana has not been evaluated till the date. Hence, its in-vitro study was conducted. Aims and objectives-To evaluate - amylase and - glucosidase activities of Amalaki Rasayana. Materials and methods- Amalaki Rasayana was prepared with 16 times levigation (Bhavana) of fine powder of fruit pulp of Indian gooseberry (Amalaki, Emblica officinalis Gaertn.) with its fresh fruit pulp juice equal in quantity for each levigation leading to 36.33% of powder and 63.66% of total solid content derived from 581.39% of fresh fruit pulp juice. Methanolic extract of Amalaki Rasayana was tested for Porcine pancreatic - amylase inhibition and intestinal - glucosidase activities at 4 concentrations (40, 80, 120 and 160 μg/ml) through starch as base and chromogenic DNSA (3, 5-dinitrosalicylic acid) as colouring agent, in 2 different solutions by recording absorbance with UV VIS Spectrophotometer. Results-Amalaki Rasayana exhibited comparatively better - amylase inhibition and -glucosidase inhibition by acetic acid buffer and inhibition was dose dependent at all concentration. Conclusion- Amalaki Rasayana exhibited inhibition of Pancreatic Alpha amylase and intestinal alpha glucosidase in dose dependent manner in the concentration of 40, 80, 120 and 160 μg/ml thus have potential to reduce post prandial hyperglycemia.

Keywords: Alpha amylase, Alpha glucosidase, Emblica officinalis, Amalaki Rasayana.