Abstract

HUMORAL IMMUNOLOGICAL RESPONSE TO CYTOMEGALOVIRUS IN CONGENITALLY INFECTED NEONATES, INFANT AND REAL-TIME PCR QUANTIFICATION OF HUMAN CYTOMEGALOVIRUS DNA FOR DETECTION VIRAL LOUD IN HILLA CITY, IRAQ

*Dr. Zainab Khudhur Ahmad Al-Mahdi, Dr. Ammar Abass Shalaan,Dr. Nada Khazal Kadhim Hindi, Nada Khalid Ahmad , Sahar Ahmad Ibraheam.

Abstract

Aim of the Study.The current study was conducted to use the in vivo model of congenital CMV to examine the humoral immune responses in vertically infected neonates and infants as well as estimate CMV DNA viral loud. Methods: Both CMV-specific IgM and IgG were tested by VIDAS.Viral loud was measured for new born babies for test group and for adult as control group, the copy number of immediate early gene was estimated by real time PCR. Samples was taken from Babylon maternity and paediatric hospital from May. 2012 to Jan. 2013. Result: Study showed that all neonates and infant were CMV IgG positive while few cases show both CMV specific IgM and IgG (13%) this because IgM antibodies generally do not cross the placenta during normal pregnancy, very few cases show just IgM(9%). CMV DNA viral load was measured using real time PCR, new born babies show higher CMV DNA than Adult patients this result reflect the important of IgM for viral neutralization. Conclusion: It was very important of checking and screening women in child bearing age to prevent horizontal congenital infection. IgG cannot prevent the CMV infection in neonate and infant, CMV IgM was detected in few newborn babies, CMV Viral loud in newborn babies was higher than in infected adult can reflect the important of CMV IgM for viral neutralization. Quantitative PCR can establish for assessment of neonate, infant damage and prognosis of infection and CMV RT PCR can use as alternative method for CMV detection.

Keywords: congenital, CMV IgM, CMV IgG. CMV, DNA.