Abstract

COMPARATIVE EVALUATION OF THE FLAVONOIDS CONSTITUENTS IN SOME VERBENAEUS SPECIES CULTIVATED IN EGYPT.

Hesham M. A. Ali, Gouda T. M. Dawoud*, Atef A. El-Hela and Emad A. Ewais

Abstract

Phytochemical screening of Tectona grandis (1), Verbena bipinnatifida (2), Duranta repens (3), Duranta lorentzii (4), Caryopteris incana (5), Gmelina arborea (6), Gmelina hystrix (7) and Verbena hybrida (8) plants showed the presence of flavonoid compounds. Quantitative analysis of total flavonoid compounds using Aluminium chloride reagent revealed the presence of (42.80., 74.10, 67.20, 96.20, 112.80, 27.40, 32.40 and 57.60 μg Quercetin Equivalent (QE) per 100 g dried sample weight), respectively. HPLC analysis of flavonoids revealed the presence of 24 peaks all of them were qualitatively identified and quantitatively estimated. The flavonoids were represented as five C -glycosyl flavones, four flavones, eight flavonol glycosides, five flavanones and two 2,3 dehydro flavone. Luteolin-6-arabinose-8-glucose was a major component of plants 3,4,5 and 6 (1206.81, 2487.41, 1464.29 and 1570.66), While Apigenin-6-arabinose-8-galactose was a major component of plants 4 and 5 (403.95, 223.59), Apigenin-6- rhamnose-8- glucose was a major component of plant 3 (785.24), Apigenin-6-glucose-8-rhamnose was a major component of plant 8 (480.46), Luteolin-7-O- glucose was a major component of plant 4 (299.89), Narengin was a major component of plant 5 (5787.49), Hesperdin was a major component of plants 2,3, 4,5,7 and 8 (3826.90, 1024.50, 1568.32, 1501.16, 1113.65 and 1104.40), Rutin was a major component of plants 3 and 4 (636.30, 886.74), Kaempferol - 3,7 - dirahmnoside was a major component of plant 1 (283.87), Quercetrin was a major component of plant 3 (505.12), Quercetin was a major component of plant 4 (332.82), Kaempferol-3-(3-p-coumaroyl) glucose was a major component of plant 8 (967.52), Hespertin was a major component of plant 5 (341.38), and Acacetin was a major component of plant 2 (1005.04).

Keywords: Verbenaceae, Flavonoid, AlCL3, HPLC.