Abstract

ISOLATION AND CHARACTERIZATION OF LIPASE PRODUCING BACTERIA FROM RESTAURANT WASTE WATER

V. P. Sutar and J. V. Kurhekar*

Abstract

The presence of Fats, Oils and Greases (FOGs) in restaurant waste water is a major problem. It is not only responsible for increased BOD and COD but also causes a number of environmental problems. In this study, five lipolytic bacteria, Staphylococcus petrasii subsp. jettensis, Staphylococcus epidermidis, Acinetobacter junii, Pseudomonas composti and Pseudomonas aeruginosa were isolated from a local restaurant waste water. The isolates were identified using morphological, physiological and biochemical tests. The 16 S rRNA analysis was performed for species level identification. The bacterial isolates were checked for their potential to secrete enzyme Lipase in mineral medium supplemented with various vegetable oils, used in restaurants. The maximum production of lipase (more than 6 Units/ml) was observed in presence of soybean, sunflower and cotton seed oil. The ground nut oil and palm oil showed comparatively less lipase production.

Keywords: Restaurant waste water, Lipolytic bacteria, Fats, Oils and Greases (FOGs), Lipase.