Abstract

ANTI-FERTILITY POTENTIAL OF METHANOLIC EXTRACT OF (HENNA) LAWSONIA INERMIS (LYTHRACEAE) ROOT IN FEMALE RATS

Priyanka Singh* and Dr. Shashi Bhooshan Tiwari

Abstract

Background: Methanolic extract of Lawsonia Inermis root (L.Inermis) has been used as an anti-fertility plant in India. Objective: To investigate the effects of L.Inermis on the fertility rate in female rats. Material and Methods: In this experimental study, female albino rats were divided randomly into five groups according to pharmacological screening models. Group 1 was taken for investigating the preimplantation activity, group 2/ for investigating anti implantation activity, 3/ for investigating of biochemical parameters, 4/ for the Teratogenic activity and 5/ for the body weight and uterus weight. In these groups, the animals were divided into three groups as: 1. Control group received distilled water 2. Treated group received plant extract at dose of 100mg/kg of body weight 3. Treated group received plant extract at dose of 200 mg/kg on the 1st day pregnancy according to the model. In group 1, treated animals were euthanized at 7th days of pregnancy and number of implantation sites was counted. In group 2, treated animals maintained till delivery time and after delivery, the number and weight of neonates were investigated. Results: Data showed that administration of L. Inermis extract on days 1-5 of pregnancy significantly decreased the number of implantation sites, number and weight of neonates. Conclusion: The results of present experiment suggested that the methanolic extract of root of L. Inermis exerted a significant anti-fertility effect in female rats. The dose and efficacy of results could be extrapolated in future clinical trials.

Keywords: Anti-fertility, Lawsonia Inermis.