Abstract

GENOTOXICITY EVALUATION OF PEO-PPO BASED STAR BLOCK COPOLYMER T1107 AND MICELLE ENCAPSULATED ANTIOXIDANTS BY CYTOKINESIS-BLOCK MICRONUCLEUS (CBMN) ASSAY

Urjita Sheth*, Meonis Pithawala and R. Krishnamurthy

Abstract

In the present study, the in vitro genotoxicity of star shaped polyethylene oxide-polypropylene oxide (EO-PO) branched octablock copolymer Tetronic®1107 (T1107; mol.wt.: 15000 g.mol-1) was assessed in human lymphocyte culture using CBMN assay. The lymphocytes were exposed to free and micelle encapsulated antioxidants viz. curcumin (25 μg/ml) and quercetin (15 μg/ml) for 48 h and analysed by the CBMN assay. The micellar drug formulations were prepared in 15% aqueous solution of T1107. There were no statistically significant differences in the frequencies of MNBNCs between untreated and treated cells in either of the treatment used. This suggests that the block copolymer did not have potential genotoxicity.

Keywords: Genotoxicity, CBMN, Tetronics®1107, Curcumin, Quercetin.