Abstract

ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF FLAVONOIDS FROM NYCTANTHES ARBORTRISTIS AND TYLOPHORA INDICA

Deepika Harit* and R. A. Sharma

Abstract

There are a numbers of bioactive compounds in plants, such as alkaloids, tannins, flavonoids, sterols, triterpenes, etc., noted to have the major role in nutrition, physiology and control of diseases. Flavonoids constitute one of the most characteristic classes of compounds in higher plants. The foremost important task in this paradigm is the screening of these compounds in the plants. The chromatographic study of the compounds serves to be a very useful and reliable source in the process of bioactive compounds screening in plants. According to the ethnobotanical information, it has been reported that both the plants Nyctanthes arbortristis and Tylophora indica possesses some medicinal importance. Hence in the present study, an attempt has been made to identify the flavonoid constituent in both the plants using TLC and PTLC. Further, the isolation of the same compound using the standardized solvent system viz., Benzene: Acetic Acid: Water (125:72:3). The confirmation of the isolation was done by IR Spectroscopy and GC- MS analysis.

Keywords: Flavonoids, Nyctanthes Arbortristis, Tylophora Indica, TLC, IR, GC-MS.