Abstract

EFFECT OF SILVER NITRATE ON CALLUS CULTURES OF SPILANTHES ACMELLA MURR - AN ENDANGERED TOOTH ACHE MEDICINAL HERB

Sujana Papani* and Saivenkatesh Korlam

Abstract

Efficient protocols for callus production from Spilanthes acmella were evaluated in the presence of ethylene antagonist AgNO3 in a callus inducing M S media supplemented with various concentrations of plant growth hormones ( IAA, 2,4-D, NAA and BAP). Addition of 0.2, 0.4 & 0.6 mg/L AgNO3 to the M S media significantly increased regeneration frequency and also produced more organogenic callus when compare to non-supplemented media. Among various concentrations administrated 0.2 and 0.4 mg/L AgNO3 were found to be most promising in producing 100% callus. Callus inducing media supplemented with 1.0mg/L NAA, 0.1 mg/L BAP And 0.4 mg/L AgNO3 found to be best suitable to produce green organogenic callus from hypocotyl explants of Spilanthes acmella. Administering M S media with high concentration of Auxins (2, 3 mg/L) promoted root formation rather than callus initiation.

Keywords: Callus, Ethylene inhibitor, AgNO3, Asteraceae, Spilanthol, Auxins.