Abstract

EVALUATION OF ANTIOXIDANT ACTIVITY IN POLYHERBAL EXTRACT

*Chaitanya Sravanthi Kota, Dr. Hemanth Kumar V. and Dr. Sunitha Reddy M.

Abstract

The reaction of oxidation is universally important reaction taking place in living and materialistic matter. Oxidation is the key reaction happening in the universe. Even though it is important in the cell life as well as non biosystems, yet times it might be unwanted reaction in some cases. One such case is release of more number of free radicals in a reaction in the biocell may lead to many unwanted cascade of reactions in the basic unit of life. These cascade of reactions may further give rise to reactions that cause cell damage in turn affecting the tissues of the organs and finally functioning of the organ. The present study was conducted to evaluate the antioxidant activity of the Polyherbal extract. The antioxidant activity was evaluated by following DPPH assay method. The standard used in this method was ascorbic acid.IC50 calculated from the data of the absorbance and percentage Inhibitory activity of Polyherbal extract was recorded as 20.06mcg/ml concentration where percentage Inhibitory activity of ascorbic acid was calculated as 26.74mcg/ml.

Keywords: Antioxidant activity, DPPH, Polyherbal, Oxidation.