Abstract

SYNTHESIS, IN VITRO ANTICANCER AND MOLECULAR DOCKING STUDIES OF NEW BISISATINS AS ANTICANCER AGENTS

Ravi Jarapula, Sriram Rekulapally, Manasa Cidda and *Sarangapani Manda

Abstract

Fifteen symmetrical bis-schiff base of isatin derivatives were synthesized by reaction of succinic acid hydrazide with various isatins and the synthesized compounds were characterised by spectral analysis. The substances were further subjected to in vitro cytotoxicity evaluatation against A549 and MCF-7 cell lines with MTT assay. All the synthesized compounds shown significantly inhibited the growth of MCF-7 cells over the A549 cells and the IC50 values of all the compounds were found between 10.24 and 28.65 μM. The compound 6j has resulted highest cytotoxicity in the entire series studied, in addition 6d, 6c, 6e and 6n were shown to display moderate activity. Further, molecular docking studies of the ligand’s were done on EGFR using GRIP batch docking method. The compounds 6c, 6d, 6e, 6i, 6j and 6l exhibited good docking (PLP) scores with receptor having Hydrogen, Hydrophobic and Vander Waal’s interactions.

Keywords: Anticancer; Isatin; Molecular docking; Succinohydrazide; MTT Assay.