Abstract

EVALUATION OF TRANSFORMING GROWTH FACTOR-BETA 1 (TGF-β1) LEVELS IN GCF AND SALIVA DURING INITIAL ORTHODONTIC TOOTH MOVEMENT – AN IN VIVO STUDY

Dr. Femin Pushpan Karyat*, Dr. T. Shobbana Devi, Dr. Valai Kasim Shakeel Ahmed,
Dr. N. R. Krishnaswamy

Abstract

Aim: To determine the changes in concentration levels of TransformingGrowth Factor-β1 (TGF-β1) as a reaction to light continuousorthodontic forces during initial stage of alignment in GingivalCrevicular Fluid (GCF) and saliva and also to compare the expressionlevels of TGF – β1 in GCF and saliva. Materials and Methods: GCFand saliva samples were collected from ten healthy subjects (6 femalesand 4 males, aged 13-23yrs). The samples were collected at thefollowing stages:- T0 – Pre Treatment, T1 – 7 days after initiation oforthodontic treatment, T2 - 30 days after initiation of orthodontictreatment, disposable micropipettes were used for GCF collection and the saliva samples werecollected in sterile containers. The collected samples were stored in sterile Eppendorf tubes at -80°C until subjected to ELISA test to determine the concentrations of TGF – β1. Results: Themean concentration levels of TGF-β1 in GCF was 25.8 + 15.6 pg/ml at T0, 32.5 + 16.1 pg/mlat T1 and 22.3 + 11.4 pg/ml at T2. The mean salivary concentration levels of TGF-β1 was 22.2+ 12.2 pg/ml at T0, 30.1 + 15.8pg/ml at T1 and 35 + 13.2 pg/ml at T2. The comparison betweenGCF and salivary levels of TGF-β1 (Intergroup) did not show any statistically significantdifference. Conclusion: The light continuous forces applied during initial stage of alignmentbrought about changes in the levels of TGF-β1 expression in both GCF and Saliva. There is apositive indication that saliva could be used as a viable substitute for GCF when used forassessing bone remodeling in patients undergoing orthodontic treatment.

Keywords: Orthodontic tooth movement; Gingival crevicular fluid; Saliva; TGF-?1.