Abstract

SPECTROPHOTOMETRIC DETERMINATION OF OXYFEDRINE IN TABLETS

*E. Mallapu Rani

Abstract

The present cerimetric method is a novel spectrophoto metric method for the assay of oxyfedrine in pharmaceutical formulations. The proposed new spectrophotometric method is based on a charge transfer complexation reaction of heterocyclic ring containing drug. In this method, the oxidation of oxyfedrine drug by a known excess amount of cerium IV sulphate in acid medium, and then unreacted cerium IV sulphate was treated with iron II sulphate, After 5 minutes the resultant iron III sulphate, solution was treated with (1M) Ammonium thiocyanate, immediately if forms blood red colour of iron IIIsulphatethiocyanate drug complex. This blood – red colored complex formed under standardized conditions was, measured at 560 nm against reagent blank, The drug calibration graph was obtained by plotting absorbance values against the concentration of oxyfedrine drug solution. The calibration graph was found to be linear over the concentration range of 50 – 250 g/ml for oxyfedrine. The linearity of the curve obtained indicates that it obeys Beer’s law. Results of analysis were validated statistically and by recovery studies. The procedures described were successfully applied to the determination of oxyfedrine in tablets.

Keywords: Oxyfedrine spectrophotometric cerium IV sulphate, Iron III sulphate (ammonium ferrous sulphate solution) Ammonium thiocyanate solution.