Abstract

U-HPLC (ULTRA-HIGH-PRESSURE LIQUID CHROMATOGRAPHY) SEPARATION OF INDOLE ALKALOID STRYCHNINE

Pasupuleti Sreenivasa Rao*, Gangapatnam Subramanayam and Matcha Bhaskar

Abstract

Ultra-high-pressure liquid chromatography (UHPLC) is an updated version of HPLC platform. It is a modern tool, useful for quick analysis, in short time and its column equilibration, is apt for method development. Strychnos genus (Loganiaceae) is cosmopolitan in distribution and many species related to it commonly used in several native medical practices. The majority of the members of Strychnos genus share similar chemical composition and contain common indole alkaloids. Purification and separation of Indole alkaloids from few species of Strychnos is already reported. In one study we also reported purification and quantification of Indole alkaloids by isocratic RP (Reverse Phase)-HPLC and further structural elucidation by IR (Infrared), 1H-NMR(Nuclear magnetic resonance),13C-NMR and LC –MS (Liquid Chromatography and Mass Spectroscopy) spectral analysis. However for the first time, we report the separation of the Indole alkaloid strychnine by U-HPLC. A simple and highly efficient RP-HPLC method was developed for the detection of alkaloid strychnine and also useful for qualitative and quantitative analysis. After optimization of the separation conditions, the indole alkaloid strychnine will be separated within 6 min and quantified with high sensitivity.

Keywords: Alkaliods, HPLC, TLC, Natural products, Strychnos.