Abstract

ESTIMATION OF QUERCETIN FROM PEEL OF UNRIPE FRUIT OF TRICHOSANTHES ANGUINA, LINN. BY REVERSE-PHASE HIGH PERFORMANCE CHROMATOGRAPHY

Deven Bhupendra Metha*, Sachin Uttam Rakesh, Priyanka Patil

Abstract

Several polyphenols have been tested in Trichosanthes anguina, Linn. The flavonoid content is important because of the pharmacological properties of these compounds, whereas quercetin has been proved to be an anti-oxidant, anti-inflammatory and hepatoprotective compound. A reversed-phase HPLC method has been developed and applied to determine quercetin content in hydroalcoholic extract of dried flowers of Trichosanthes anguina, Linn. in a single analysis. The quercetin was analysed with a HiQ Sil C-18 column by isocratic elution using 0.01% (v/v) phosphoric acid–acetonitrile as the mobile phase. The flow rate was 1.2 ml min−1, and detection was set at 265 nm. The recovery of the method was in the range of 98.50–99.40%, and all the compounds showed good linearity (r > 0.98332) in a relatively wide concentration range.

Keywords: Trichosanthes anguina Linn.; Quercetin; RP- HPLC.