Abstract

A COMPARATIVE STUDY OF MALE GONADAL ACTIVITY IN MANGIFERIN INDUCED RATS VIS – A – VIS CRUDE BARK EXTRACT OF MANGIFERA INDICA

Tasneem Khandekar, Chaitali Bose, Indrani Chakraborty, Subhasis Ghosal and Nirmal Pradhan*

Abstract

Mango tree (Mangifera sp.) is well known economic plant and its bark extract is mostly important in the field of pharmaceutical research and clinical applications. In the present study the ethanolic crude extract of mangifera bark (mother/tincture) at the dose of 0.1 ml/100gm b.wt and parallel the pure mangiferin at the dose of 1mg/0.1 ml/100gm b.wt./day has been studied in male rats for 14 days consicutively. The drugs were administrated i.p parallel with the vehicle (70% alcohol) control. The results showed no change in relative weights of testes, but the weights of ventral prostate and seminal vesicle were significantly reduced than control. The epididymal weight of pure treated group was increased significantly in comparison to extract treated group. The biochemical studies of ascorbic acid content in testes & liver was significantly reduced in all treated groups when compared to control but was increased in pure treated group but in epididymis it was significantly elevated in pure treated group in compare to control and extract treated group. The serum cholesterol level showed no change at all. The testicular cholesterol also followed the same trend but was increased in pure group in comparison to extract treatment. Again the fructose level in prostate was significantly reduced in all the treated groups, but in seminal vesicle, only a reductive change was found in pure treated group when compared to control. The SEM study supported also the above findings. As degeneration of spermatogenic cells in testes (qualitative study) were predominant in extract treated group than pure drug treatment in comparison to control.

Keywords: Mangiferin, Mangifera indica, Bark extract, Male Antifertility, Spermatogenesis.