Abstract

A BRIEF REVIEW ON ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY

Sathe Komal Pramod* and Khedkar Amol Navnath

Abstract

Ultra Performance Liquid Chromatography (UPLC) is a relatively new technique. It is an analytical method commonly used in analytical chemistry and in the pharmaceutical industry. Its operation is based on column chromatography that is often used to investigate mixtures. This technique gives new possibilities in liquid chromatography. The use of which decreases the length of column, saves time and reduces solvent consumption. It shows a great enhancement in speed, resolution and sensitivity of analyses by using particle size less than 2μm. The quality analyses of various pharmaceutical formulations are transferred from HPLC to UPLC system. The separation on UPLC is performed under very high pressure (up to 100MPa). Separation efficiency remains maintained or is even improved by UPLC. This review introduces the theory of UPLC.

Keywords: Chromatography, UPLC, Resolution, HPLC.