Abstract

PHYTOCHEMICAL ANALYSIS AND ANTI-OXIDANT ACTIVITY OF NOTHOPODYTES NIMMONIANA (GRAH.) MABB ROOT AND LEAF

Priyanka Rao*, Nataraj H. R. and Subba Rao V. Madhunapantula

Abstract

Oxidative stress in human body contributes to the pathogenesis of many human diseases. Antioxidants are found naturally in plant materials and also in supplements. Due to their natural origin, the antioxidants obtained from plants are of greater benefit in comparison to synthetic one. Nothopodytes nimmoniana (J. Graham) (Icacinaceae), commonly known as “Narakhya” been used for the treatment of cancer in villages. Camptothecin (CPT), a monoterpene indole alkaloid, present in the drug is regarded as one of the most promising anticancer drug of the 21st century. However, it is currently unknown which phyto-constituent is responsible for anticancer activity. Therefore, an attempt is made to fractionate and isolate active ingredient(s) from this plant, Experimentally, first, the root and leaf of Nothopodytes nimmoniana were extracted sequentially using hexane, dichloromethane, chloroform, aqueous ethanol (70% ethanol) and water. Next, characterisations were done by quantitative analysis for Total Carbohydrates, Glucose Oxidase, Reducing sugars, total phenols and Antioxidant Assay. The data showed a significant fraction with Antioxidant activity with Chloroform being the most effective fraction.

Keywords: Nothopodytes nimmoniana, phyto-constituent, anti-cancer activity.