Abstract

DYE DEGRADATION BY MICROORGANISMS

Narsinge A. P.*

Abstract

In the present study, an attempt was made to isolate, identify the dye degrading bacteria. A textile dye degrading bacterium was isolated from dye contaminated soil from Ichalkaranji. The dye degrading bacterium was identified as Bacillus licheniformis sp. F4 by 16S rRNA sequencing. The ability of this isolated bacterium for the efficient degradation of textile dye reactive golden yellow was evaluated. The effects of various physicochemical parameters such as pH, temperature, initial dye concentration, inoculum, carbon sources were examined for maximum degradation of textile dye reactive golden yellow. The isolated bacterial culture degraded reactive golden yellow (100 mg/l) by 80% after 24 hours of incubation period at optimum pH 7 and temperature 300C under shaking condition. A 15% (v/v) inoculum and 1% (w/v) glucose concentration were found to be optimum for degradation of textile dye reactive golden yellow. The biodegradation of reactive golden yellow was monitored by UV-Visible spectroscopy. The GC-MS analysis confirmed the biodegradation of textile dye reactive golden yellow. The result shows that the isolated bacterial culture has good potential in the degradation of textile dyes from textile waste water under aerobic conditions.

Keywords: Textile Dye, Degradation, Microorganisms.