Abstract

ISOLATION AND CHARACTERIZATION OF SOME ANTIBIOTIC RESISTANT BACTERIA FROM HOSPITAL DRAINAGE SAMPLE

Dr. Anandhi K.*

Abstract

Hospital waste possesses a significant impact on health and environment. In this present study hospital waste dumped soil sample was collected from private hospital at Tiruchirappalli Dt. and analysis the bacterial population in the soil. Again the soil samples were enriched onto nutrient broth medium incorporated with an antibiotic Streptomycin (200mg/100ml) for the screening of antibiotic resistant bacteria present in the soil. Colonies developed on the plates were identified using standard manual. Based on the morphological and biochemical characters, six bacterial species were isolated and identified namely Escherichia coli, Enterobacter aerogenes, Pseudomonas aeruginosa and Staphylococcus aureus. All the four isolates were again screened for their susceptibility against ten different antibiotics. Among the isolates tested, Escherichia coli and Staphylococcus aureus showed maximum resistance against most of the antibiotics followed by Enterobacter aerogenes. Andrographis paniculata, Leucas aspera and Ruellia tuberose plant leaves were collected and phytochemical compounds were analysed in the plant extract. Among the study maximum compounds were present in acetone and methanol extracts. In this study Andrographis paniculata plant leaves maximum antibacterial activity was recorded against isolated all antibiotic resistant bacteria. From this study, it can be said that there is an urgent need to raise awareness and education on medical waste issues. Proper waste management strategy is needed to ensure health and environmental safety.

Keywords: Antibiotic resistance, phytochemical compounds, Andrographis paniculata, Leucas aspera and Ruellia tuberose.