Abstract

GENETIC POLYMORPHISM AND SERUM LEVEL OF VASCULAR ENDOTHELIAL GROWTH FACTOR ASSOCIATED WITH FIRST TRIMESTER PREGNANCY OUTCOME IN ICSI PROGRAM

Luma Waad Abdul Alteef*, Dr. Salwa Jaber Al-Awadi, Dr. Manal Taha Al-Obaidi

Abstract

VEGF plays an important role in normal physiological processes that require increased vascularization to bring oxygen and nutrients to tissues, including embryonic development. This study determine the relationship between changes in serum level of vascular endothelial growth factor (VEGF) protein and pregnancy outcome in women undergoing ICSI and also the relationship between (VEGFA) single nucleotide polymorphism (rs 3025039) 936 C/T and it’s incidence on pregnancy outcome after ICSI. The study was done on 51 infertile women and 34 fertile women with at least 2 live births. Patients categorized as pregnant, non-pregnant and miscarriage. The levels of vascular endothelial growth factor protein were measured by ELISA, the results showed that the levels of vascular endothelial growth factor in control, ICSI patients, pregnant, non-pregnant and miscarriage were (1.99±0.54mg/ml, 2.46±0.64mg/ml, 2.80±0.61mg/ml, 2.21±0.60mg/ml, 2.56±0.30 mg/ml respectively). The significant increase shown in pregnant when compared with control, pregnant p= 0.0004, (p<0.05) and also there is significant increase in pregnant when compared with non-pregnant, p=0.0081, (p<0.05). The genetic polymorphism VEGFA/rs 3025039 was detected by specific primers. Results of amplification showed that a DNA fragment 198 bp was obtained after electrophoresis on 2% agarose gel. The nucleotide sequence of each amplified fragment was determined. Results of sequencing showed that the genotype frequency in control were CC (85.29%), CT (11.76%), TT (2.94%), in ICSI patients were CC (72.54%), CT (23.52%), TT (3.92%), in pregnant were CC (62.5%), CT (16.66%), and TT (0%), in non-pregnant were CC (70%), CT (25.92%),TT (3.7%) and in In miscarriage were CC (60%), CT (20%),TT(20%). VEGFA/rs 3025039 polymorphism has a non-significant association with pregnancy outcome in ICSI program. The results of this study shows that increasing level of vascular endothelial growth factor associated with pregnancy also there is no association between 936 C/T polymorphism and pregnancy, implantation failure and miscarriage.

Keywords: Vascular Endothelial Growth Factor (VEGF), rs3025039, ICSI.