Abstract

α- AMYLASE INHIBITORY ASSAY OF DIFFERENT FOLIAR EXTRACTS OF ANTHOCEPHALUS CADAMBA (ROXB.) BY USING DNSA METHODS

Basant Kumar*, Abhishek Kumar and Garima Bartariya

Abstract

Anthocephalus cadamba is a medium size plant belongs to Rubiaceace family and found in all over in India. Many studies suggest herbal remedy by cadamba to treat diabetes or overcome blood glucose level. Diabetes is a metabolic disorder syndrome. A therapeutic approach for treating diabetes is to decrease the carbohydrates hydrolyzing enzyme such as α-amylase. α-amylase is a major digestive enzyme, which hydrolyze the 1,4- α-linked glycosidic bond of polysaccharides and converts it into disaccharides and trisaccharides. Three different solvents such as petroleum ether, ethanol and distilled water were used for the extraction using soxhlet method and obtained extract were used for the α-amylase inhibition assay. α-amylase inhibition assay was performed by using DNSA i.e 3,5- dinitro salicyclic acid. The absorbance was recorded at 540nm by using UVspectroscopy (PerkinElmer). Result of this study revealed that leaves extract showed α- amylase inhibition in a dose dependent manner. Three different concentrations i.e 2.5, 5, 10 mg/ml of each extract was used for the present study. The extracts showed maximum inhibition at a concentration of 10 mg/ml which is increased with increasing concentration. The prepared extract showed significant inhibition of α- amylase at all the selected concentration i.e 2.5, 5, 10 mg/ml with the increase in concentration.

Keywords: ?-amylase, Polysaccharides, Anthocephalus cadamba, DNSA Method.