Abstract

EFFECT OF QUINALPHOS ON BIOCHEMICAL STUDIES IN FRESH WATER FIELD CRAB, SPIRALOTHELPHUSA HYDRODROMA

S. Pandiammal*, J. Manju Bashini and P. Senthilkumaar

Abstract

The effect of quinalphos pesticide on nutritional parameters (protein, carbohydrate and lipid) in the tissues (muscle, gills, hepatopancreas, spermatheca and ovary) of Spiralothelphusa hydrodroma was determined in a toxicity test. The LC50 values were obtained by probit regression line, taking test concentration and corresponding percent mortalities on log value and probit scales respectively. Sublethal studies are helpful to assess the response of the test organism to stress caused by pesticides. S. hydrodroma were exposed to varying sublethal concentrations of quinalphos (24h, 48h, 72h and 96h). Chronic time course studies on the effects of pesticide were conducted by exposing to sublethal safe concentrations for 24 hours. At the end of the treatment period the control and treated crabs were dissected and tissues namely, muscle, gills, hepatopancreas, spermatheca and ovary were collected for biochemical studies.

Keywords: Spiralothelphusa hydrodroma, tissues, pesticide, nutritional studies.