Abstract

IN-VITRO AND IN VIVO ANTI-INFLAMMATORY POTENTIAL OF SILVER NANOPARTICLES SYNTHESIZED FROM CALOTROPIS GIGANTEA LATEX EXTRACT

Pralay Maity, Madhubanti Bepari, Ananya Pradhan, Rathindranath Baral and Sujata Maiti Choudhury*

Abstract

In recent years plant mediated biological synthesis of nanoparticles isgaining importance due to its simple synthetic procedure and ecofriendliness.The present study describes in-vitro and in vivo antiinflammatoryactivitiesof biogenic stable silver nanoparticlessynthesized by using ethanolic extract of Calotropis gigantea latex (AgNPCGs). The silver nanoparticles were previouslycharacterized byUV-visible spectroscopy, dynamic light scattering (DLS), zetapotentialmeasurements, Fourier transform infrared spectroscopy andtransmission electron microscopy. The dose-dependent antiinflammatoryactivity of silver nanoparticles was assessed by humanred blood cell membrane stabilization, inhibition of proteindenaturation, proteinase and lipoxygenase inhibitory action. Thepercentage of HRBC membrane stabilization of Ag NPCGs at 500μgml-1 was found to be 75.28%. At the concentration of 500 μg ml-1, AgNPCGs showed 74% proteinase inhibitory action. Lipoxygenaseactivity was inhibited by Ag NPCGs at the concentration range of 50-500μg ml-1. Ag NPCGsalso exhibited better percentage of inhibition in the carrageenan induced right hind pawedemathan that of standard drug indomethacin. On the basis of present study, it is concludedthat Ag NPCGs possess potent anti-inflammatory activity.

Keywords: Silver nanoparticles, Calotropis gigantea, Anti-inflammatory activity, Protein denaturation.