Abstract

PREPARATION AND OPTIMIZATION OF ROSUVASTATIN CALCIUM LOADED SOLID LIPID NANOPARTICLES BY CENTRAL COMPOSITE DESIGN

Harjeet Singh*, Ram Dayal Gupta and Girendra Gautam

Abstract

Background: Solid lipid nanoparticles are an alternative carrier system used to load the drug targeting, to improve bioavailability by increasing solubility, permeability and protecting the drug from first pass metabolism. Objective: The aim of this investigation was to design and evaluate solid lipid nanoparticles (SLNs) of rosuvastatin calcium. Materials and methods: A modified solvent emulsification diffusion technique was used to produce the rosuvastatin loaded solid lipid nanoparticles. A 4-factor, 3-level Central Composite design was applied to study the effect of independent variables (factors) i.e. lipid (A), surfactant concentration (B), stirring speed (C) and drug amount (D) on dependent variables (responses) i.e. drug entrapment efficiency (Y1), drug loading (Y2) and particle size (Y3). 3-D surface response plots were drawn and optimized formulation was selected based on desirability factor. Results: The results of optimized formulation showed average particle size of 115.4 nm, entrapment efficiency of 97.16% and drug loading of 60.34%. Transmission electron microscopy (TEM) reveals that particles were spherical in shape with smooth surfaces and uniform distribution. Conclusion: Thus, the present study can be useful for the successful design, development and optimization of SLNs for rosuvastatin calcium using a 4-factor, 3-level Central Composite design.

Keywords: Central Composite design, Rosuvastatin calcium, Solid lipid nanoparticles, Solvent emulsification diffusion technique etc.