Abstract

ANTIBACTERIAL ACTIVITY OF ABUTILON PANNOSUM AND GREWIA TENAX LEAVES EXTRACTS

Mital K. Aadesariya, Bhagwati M. Gauni, Srinivas M. Duggirala, Vijay R. Ram*, Suhas J. Vyas

Abstract

Plants have been proven to be a rich source of diverse class ofantimicrobial molecules useful for treatment of various infections.These purified or crude antimicrobial constituents are obtained fromplants by extraction using a range of organic solvents. Theirantimicrobial or inhibitory effects on various microbes can be checkedby agar well diffusion method. In this study we have focused onassessment of antibacterial activity of leaf extracts of Abutilonpannosum (APL) and Grewia tenax (GTL) using four differentsolvents i.e., Milli-Q, methanol, petroleum ether and isopropanol bythe agar well diffusion method. The plant extracts were prepared bysuccessive extraction method and tested against eleven different humanpathogenic bacteria. The test organisms included Gram-positive organisms such asStaphylococcus aureus, Enterococcus fecalis, Streptococcus epidermidis, Streptococcuspneumoniae and Gram-negative organisms; Escherichia coli, Salmonella typhi, Serratiamarcescens, Klebsiella pneumoniae, Pseudomonas aeruginosa and Proteus vulgaris. Varioussolvent extracts of both plant materials prepared at an initial concentration of 1 mg/mLshowed variable degree of inhibitory activity against tested pathogens. The results showedthat both the plant extracts have very good inhibition against S. pneumoniae and S. typhi,moderate inhibition against E. fecalis, S. epidermidis, and P. aeruginosa while they could not inhibit the growth of E. coli, S. aureus, S. marcescens, K. pneumoniae, B. subtilis and P. vulgaris. The results were also compared with standard antibiotics for Gram-positive and Gram-negative organisms.

Keywords: Antibacterial activity, Grewia tenax, Abutilon pannosum, Agar well diffusion.