Abstract

DESIGN OF A VALIDATION OF CAUDAL ANALGESIMETRY IN EXPERIMENTAL PHARMACOLOGY

Adouani Badr*, Faouzi My Abbas, Cherrah Yahia and Meddah Bouchra

Abstract

Caudal analgesia is one of the most used tests in recent years to evaluate the reflex response to nociceptive stimulation in the animal model. The aim of our work was to study the different parameters involved in the analgesic effect of a pharmacological substance when performing a tail-flick test. The device used is a Tail-flick meter LE 7106 PANLAB (part of the Harvard Bioscience Family Spain), our animal model was composed of "wistar" rats and "swiss" mice. The analgesic substance used for the control lot was acetylsalicylic acid; the dose for the intraperitoneal administration was 20 mg / kg and 100 mg / kg for the oral administration. Based on our results, there is species-specificity and sex difference for rats. Conversely, there is no sex variability for mice when it comes to response time. The mode of administration of the reference drug for peripheral analgesia does not reach the nociceptive center for the orally administered aspirin group; whereas for the intraperitoneal administered aspirin group, there is a significant difference in the focus 50, 60 and 70 compared to the control groups.

Keywords: Peripheral analgesia, tail-flick, thermal stimulation, aspirin, rats, mice.