Abstract

FORMULATION AND EVALUATION OF NANOPARTICLES CONTAINING CYCLOPHOSPHAMIDE

*Pankaj Sharma, Sarvesh Bhargava, Deepesh Parashar and Ashish Mangal

Abstract

Objectives of present study were- to maintain the therapeutic drug concentration in the site of action for a prolonged period, improve the drug’s efficiency & reduce the dose related side effect of cyclophosphamide. Cyclophosphamide nanoparticles were prepared by salting out method. Nine (F1-F9) formulations of nanoparticles were developed in research. After development of formulations, various evaluation parameters were calculated. Percentage yield of nanoparticles were determined for all 9 formulations (F1 to F9). The result for all different formulated was obtained in the range of 26.09±0.95 to 39.8±1.4. Loading efficiency was determined for all nine formulations (F1 to F9). The results for all different formulations were obtained in the range from 40.56±0.85 to 45.29±2.1. Particle size determination was done by using Malvern mastersizer instrument for the optimized formulation F8. Particle size was found 730.9 nm with maximum intensity and volume. From this data, it could be said that the nanoparticles has been formed successfully by using ionic cross linking method. Polydispersibility index of the cyclophosphamide obtained nanoparticles was found to be 0.918 and hence shows a narrow size distribution and contributes to the stability of nanoparticles. Based on the observations, formulation F8can be concluded that the PLGA coated Nano particulate targeted delivery system of cyclophosphamide formulated using widely accepted and physiologically safe polymer was capable of exhibiting sustained release properties for 8hrs.

Keywords: Nanoparticles, FTIR, Polydispersibility, Entrapment Efficiency.