Abstract

EVALUATION OF ANTIOXIDANT AND CARDIOPROTECTIVE ACTIVITIES OF BRIDELIA RETUSA ON ISOPROTERENOL INDUCED MYOCARDIAL NECROSIS IN ALBINO RATS

*Raja Sundararajan, Gopikrishna Rakam and Ravindranadh Koduru

Abstract

The present study was undertaken to evaluate the antioxidant and protective myocardial necrosis effects of extracts of Bridelia retusa in isoproterenol induced rats. Different concentration of chloroform, ethyl acetate and methanol extracts of Bridelia retusa (Euphorbiaceae) were investigated for in-vitro antioxidant activity by thiocyanate method. The methanol extract of Bridelia retusa exhibited maximum inhibitory activity of peroxidation, over the other organic extracts, which was subjected to further in-vivo antioxidant and myocardial necrosis studies. Methanol extract of Bridelia retusa was administered to rats at two different doses of 250 mg/kg and 500 mg/kg for 90 days to evaluate oxidative stress parameters like ferric reducing ability of plasma (FRAP) thio barbituric acid reactive substance (TBARS), reduced glutathione (GSH) and antioxidant enzyme levels of superoxide dismutase (SOD) & catalase (CAT). Myocardial necrosis and myocardial oxidative stress were evident in the isoproterenol (85 mg/kg, s.c, two doses at 24 h interval) administered rats for 30 days by significant rise in myocardial TBARS, myocardial GSH, plasma TBARS and plasma lactate dehydrgenase (LDH) along with reduction of myocardial SOD, myocardial CAT and myocardial glutathione peroxidase (GPX). Further myocardial necrosis was evident from the light microscopic changes. It observed that methanol extract of Bridelia retusa conferred antioxidant and myocardial protective effects by histopathological and biochemical observations in rats. It can be fulfilled that methanol extract of Bridelia retusa had significant antioxidant activity, which capacity be helpful in preventing (or) slowing the diverse oxidative stress- related diseases. Further, the results suggest that chronic administration of methanol extract of Bridelia retusa prevents oxidative stress and myocardial necrosis in isoproterenol induced in rats.

Keywords: Bridelia retusa, myocardial necrosis, isoproterenol, antioxidant, oxidative stress.