Abstract

PREPARATION AND CHARACTERISATION OF COLLAGEN/CHITOSAN/ SODIUM ALGINATE BIOCOMPOSITE ELECTROSPUN NANOFIBERS

Nandhini DP, Subashini S, Pandimadevi M*

Abstract

In the present study, Collagen (Co) ,Chitosan (Ch) and sodium alginate are used to prepare the nanofibres by electrospinning technique. Type 1 collagen is extracted from chrome containing leather waste (CCLW) and chitosan, which is extracted from prawn shells, is a natural polysaccharide, comprising a copolymer of glucosamine and N-acetyl glucosamine. Sodium alginate (SA) is an effective natural disintegrant, tablet binder and offers an attractive alternative for sustained drug-release systems. Ch-Co-SA blends are used for making the nanofibres using electrospinning technique and the surface methodology of nanofibres was observed by SEM. The TGA, DSC and mechanical properties are analysed for the electrospun nanofibers. The tensile strength and % of elongation of the biocomposite film showed high strength and flexibility, which is an important characteristic of wound dressing material. The thermogravimetry analysis was performed between 30°C and 800°C, where it showed the major loss in the mass of the biomaterial after 400°C and 69.53% (5.211 mg) of the residue remained at 800°C and the melting point was found to be 95.85°C using DSC. The electrospun Ch-Co-SA prepared can be further tested through in vitro analysis for their wound healing potential.

Keywords: Collagen, chitosan, wound healing, electrospinning, biomaterial, TGA, DSC.