Abstract

CHARACTERIZATION OF THE COMBINATION OF CHONDROTIN SULFATE, CHITOSAN AND KAPPA CARERGEENAN USING THE IONIC GELATION METHOD FOR PAINFUL KNEE OSTEOARTHRITIS

Danni Ramdhani*, Anis Yohana Chaerunisaa and Aneu Nur Utami

Abstract

Objective: Application of the use of chondroitin sulfate as an alternative medicine in addition to nonsteroidal antiinflammatory drugs has the ability to inhibit the development of osteoarthritis. The use of chondroitin sulfate in the form of nanoparticles can increase the bioavailability of the drug. This study aims to characterize the combination formula of condroitin sulfate, chitosan and kappa carrageenan. Methods: Preparation of formula consisting of 0.1% chitosan, 0.1% chondroitin sulfate and 0.05% kappa carrageenan at volume ratio of 10: 1: 1 were prepared by using ionic gelation method This method was based on the electrostatic interaction between opposite charges. Characterization of nanoparticles were performed by measuring pH, particle size, polydispersity index, zeta potential and examination of infrared spectrum. Results: The results showed that pH 3.1, an average particle size 582.9 nm, polydispersity index value of 0.324 and zeta potential value as much as -0.47 mV.

Keywords: Chondroitin sulfate, chitosan, kappa carrageenan, anoparticles, Ionic gelation, characterization.