Abstract

BACTERIAL DEGRADATION OF TEXTILE DYES BY BACILLUS SPECIES

M. Kannahi* and P. Nisha

Abstract

Rapid industrialization has necessitated the manufacture and use of different chemicals in day to day life. Water is life but now a day due to advantagement in industrialization, it is spoiling a lot. The dyes were collected from Tirupur textile industry, Tamil Nadu. Effect of physical and chemical parameters were analysed for effective degradation of the dye. B.subtilis was showed maximum activity at the pH (7), temperature (37oC) and chemical source such as carbon source (sucrose) and nitrogen source (peptone) of dye degradation. The sample were collected from dye effluent contaminated soil of Tirupur textile industry, Tamil Nadu. The serial dilution method was followed for the isolation of bacteria. The isolated bacteria were identified as Bacillus subtilis and Bacillus cereus by cultural, Gram staining and biochemical characteristics. The identified bacteria were used for decolorization of dyes by broth method. Bacillus subtilis showed maximum decolorization on red color dye (65%) and blue colour dye (48%) compared with Bacillus cereus. Bacillus cereus showed decolorization on the red colour dye (51%) and blue color dye (38%). In this study, the bacterial isolates for the decolorization of dyes was performed by broth method. After incubation the degradation of samples were analysed by UV Spectrophotometer and High Performance Liquid Chromatography.

Keywords: Textile Dyes, Bacillus subtilis, Bacillus cereus, Total solids, Total dissolved solids, Total suspended solids.