Abstract

ISOLATION AND SCREENING OF LIPASE PRODUCING BACTERIA FROM MUNICIPAL SOLID WASTES (MSW)

A. Pallavi and P. Brahmaji Rao*

Abstract

Lipases (triacylglycerol acylhydrolases EC 3.1.1.3) are hydrolysis enzyme. They hydrolyze the carboxylic ester bond through various important reactions such as trans-esterification, alcoholysis, interesterification, esterification, aminolysis. Microbial enzymes are often more useful than enzymes derived from plants or animals because of the great variety of catalytic activity. In present study, several lipase producing bacteria were isolated, from which best lipase producing isolates were further identified by means of morphological and biochemical characterization. Based on the identification characteristics and Bergy’s manual two potent isolates were screened and identified as the genus Pseudomonas (PS-4) and Staphylococcus (PS-7).

Keywords: Lipases (triacylglycerol acylhydrolases Staphylococcus (PS-7).